7-[(Substituted-thiomethyl)phenyl]acetamidocephalosporin derivatives

ABSTRACT

Novel compounds of the formula ##STR1## are useful as antibiotics.

This is a division of application Ser. No. 853,154, filed Nov. 21, 1977, now issued as U.S. Pat. No. 4,124,761.

FIELD OF THE INVENTION

This invention relates to novel cephalosporin derivatives useful as antibiotics and processes for their preparation.

SUMMARY OF INVENTION

Compounds of the following general Formula I are useful as antibiotic agents: ##STR2## WHEREIN Y is hydrogen, chlorine, bromine, a straight or branched lower alkyl group of from 1 to 4 carbon atoms, or an alkoxy group of from 1 to 4 carbon atoms; Z is a bond, oxygen or sulfur; W is hydrogen, methyl, amino, hydroxy, SO₃ H, or COOR₄ wherein R₄ is hydrogen or 5-indanyl with the proviso that when Z is oxygen or sulfur, W is other than hydroxy; R is a lower alkyl group of from 1 to 4 carbon atoms, 1,3,4-thiadiazol-2-ylthio, 5-methyl-1,3,4-thiadiazol-2-ylthio, tetrazol-5-ylthio, 1-methyltetrazol-5-ylthio, 1,3,4-oxadiazol-2-ylthio, 5-methyl-1,3,4-oxadiazol-2-ylthio, 1,3,4-triazol-2-ylthio, 5-methyl-1,3,4-triazol-2-ylthio, 1,2,3-triazol-5-ylthio, 5-amino-1,3,4-thiadiazol-2-ylthio, (4,5-dihydro-4-oxo-2-thiazolyl)thio, or cyano; R₁ is hydrogen or methoxy; R₂ is hydrogen, acetoxy, 1,3,4-thiadiazol-2-ylthio; 5-methyl-1,3,4-thiadiazol-2-ylthio, tetrazol-5-ylthio, 1-methyltetrazol-5 -ylthio, 1,3,4-oxadiazol-2-ylthio; 5-methyl-1,3,4-triazol-2-ylthio, 5-methyl-1,3,4-triazol-2-ylthio; 1,2,3-triazol-5-ylthio; R₃ is hydrogen, a cation of an alkali metal or an alkaline earth metal, ammonium or organic ammonium cations, a straight or branched lower alkyl group of from 1 to 4 carbon atoms, a straight or branched alkanoyloxy methyl group in which the alkanoyl moiety has from 2 to 5 carbon atoms, and is straight or branched, an alkanoylaminomethyl group in which the alkanoyl moiety is straight or branched and has from 2 to 5 carbon atoms and the amine nitrogen may be substituted with a straight or branched lower alkyl group having 1 to 4 carbon atoms; an alkoxycarbonylaminomethyl group in which the alkoxy moiety is straight or branched and has from 1 to 4 carbon atoms and the amine nitrogen may be substituted with a straight or branched lower alkyl group of from 1 to 4 carbon atoms, a p-(alkanoyloxy)benzyl group in which the alkanoyl moiety is straight or branched and has from 2 to 5 carbon atoms; an aminoalkanoyloxymethyl group in which the alkanoyl moiety has from 2 to 15 carbon atoms and the amino nitrogen may be mono- or di-substituted with a straight or branched lower alkyl group having from 1 to 4 carbon atoms; and pharmaceutically acceptable salts and individual optical isomers thereof.

The non-toxic acid addition salts of the compounds of Formula I such as mineral acid addition salts, for example, hydrogen chloride, hydrogen bromide, hydrogen iodide, sulfate, sulfamate and phosphate and organic acid addition salts, for example maleate, acetate, citrate, oxalate, succinate, benzoate, tartrate, fumarate, malate, mandelate, and ascorbate, are also included within the scope of this invention.

DETAILED DESCRIPTION OF INVENTION

In general Formula I the substituent group as represented by R₃ in addition to being hydrogen, a cation or a lower alkyl group may also be a alkanoyloxymethyl as represented by the structure: ##STR3## wherein R₅ is selected from a straight or branched lower alkyl group of from 1 to 4 carbon atoms; alkanoylaminomethyl or alkoxycarbonylaminomethyl as represented by the structure: ##STR4## wherein R₆ represents a straight or branched lower alkyl group of from 1 to 4 carbon atoms or a straight or branched alkoxy group of from 1 to 4 carbon atoms, and R₇ is selected from hydrogen and a lower alkyl group of from 1 to 4 carbon atoms; p-(alkanoyloxy)benzyl as represented by the structure: ##STR5## wherein R₈ is a straight or branched lower alkyl of from 1 to 4 carbon atoms; and aminoalkanoyloxymethyl as represented by the group: ##STR6## wherein m is 0 to 5, each of R₉ and R₁₀ is selected from hydrogen or lower alkyl of from 1 to 4 carbon atoms, and each of R₁₁ and R₁₂ is selected from hydrogen or a straight or branched lower alkyl group of from 1 to 4 carbon atoms.

Illustrative examples of straight or branched lower alkyl groups of from 1 to 4 carbon atoms which Y, R₅, R₆, R₈, R₁₁ and R₁₂ may represent are methyl, ethyl, n-propyl, isopropyl, n-butyl and tert-butyl.

Examples of lower alkyl groups of from 1 to 4 carbon atoms which R₇, R₉, and R₁₀ may represent are methyl, ethyl, n-propyl and n-butyl.

Examples of lower alkoxy groups which Y may represent are methoxy, ethoxy, n-propoxy and n-butoxy.

As used in reference to general Formula I the terms 1,3,4-thiadiazol-2-ylthio, 5-methyl-1,3,4-thiadiazol-2-ylthio, tetrazol-5-ylthio, 1-methyltetrazol-t-ylthio, 1,3,4-oxadiazol-2-ylthio, 5methyl-1,3,4-oxadiazol-2-ylthio, 1,3,4-triazol-2-ylthio, 5-methyl-1,3,4-triazol-2-ylthio, 1,2,3-triazol-5-ylthio, 5-amino-1,3,4-thiadiazol-2-ylthio and (4,5-dihydro-4-oxo-2-thiazolyl) this is taken to mean the following respective groups: ##STR7##

The compounds of Formula I are prepared by treatment of a derivative of Formula II with a derivative of Formula III ##STR8## wherein R, Z, W, R₁, R₂, R₃ and R have the same meaning as described in Formula and X is a halogen atom such as chlorine, bromine or iodine.

The reaction between a compound of Formula II and a compound of Formula III to give a compound of Formula I takes place when equimolar amounts of the reagents are combined in a suitable solvent. Suitable solvents include dimethyl formamide, acetone, ethyl acetate, acetonitrile, methanol, and ethanol, The temperature of the reaction may vary from 0° to 100° C. and the reaction time may vary from about 0.5 hour to 10 hours. The product of the reaction of Formula I may be obtained upon removal of the solvent or by precipitation upon combination of the reaction solution with a solvent in which the product is insoluble.

The compounds of Formula II may be used in situ without the need of prior isolation when prepared by coupling a compound of Formula IV with an acid of formula V or a functional equivalent thereof ##STR9## wherein R₁, R₂, R₃, X, Y, Z, and W are as described above.

Compounds of Formulas II and V have been described in U.S. Pat. Nos. 3,919,206; 3,948,904; 4,026,887. Compounds of Formula III are commercially available or may be readily prepared by known procedures.

The compounds of general formula IV, that is, 7-aminocephalosporanic acid and 7-aminodesacetoxycephalosporanic acid and derivatives thereof are commercially available or may be obtained from Penicillin G, cephalosporin C or cephamycin C by processes known in the art. For example compounds of Formula IV wherein R₇ is methoxy may be prepared as described by M. Sletzinger et al., J. Am. Chem. Soc., 94, 1408 (1972). Compounds of Formula IV may also be prepared as described in U.S. Pat. Nos. 3,948,904 and 4,026,887.

When the substituent group W in the above Formula V represents an amino group suitable blocking groups, for example, tert-butoxy-carbonyl, or carbobenzyloxy are employed to protect the amino function. Such blocking groups are removed after the coupling reaction by methods generally known in the art, for example, as described by Lemieux et al., in U.S. Pat. No. 3,657,232.

The preparation of a compound of Formula V wherein W is --COO indanyl may be carried out by reacting the corresponding compound of Formula V wherein W is --COOH with one mole of 5-indanol in an inert solvent such as chloroform, dichloromethane, dimethylformamide, in the presence of N,N'-dicyclohexylcarbodiimide at a pH of about 2.5 and a temperature of from 20° to 30° C. The product is isolated upon filtration of the N,N'-dicyclohexyl urea formed and subsequent removal of the solvent.

Functional equivalents of the acids as represented by Formula V include the acid halides, for example, the acid chloride, acid anhydrides, including mixed anhydrides with, for example, alkylphosphoric acids, lower aliphatic monoesters of carbonic acid, or alkyl or aryl sulfonic acids. Additionally, the acid azide or an active ester or thioester, for example, with p-nitrophenol, 2,4-dinitrophenol, or thioacetic acid, may be used, or the free acid as represented by Formula V may be coupled with the 7-aminocephalosporanic acid derivative as represented by Formula IV after first reacting the acid with N,N₁ -dimethylchloroforminium chloride or by use of a carbodiimide reagent, for example, N,N₁ -diisopropylcarbodiimide, N,N₁ -dicylohexylcarbodiimide, or N-cyclohexyl-N₁ -(2-morpholinoethyl)carbodiimide.

The coupling reaction between compounds of Formula IV and Formula V is generally carried out in the presence of a solvent such as ethyl acetate, acetone, dioxane, acetonitrile, chloroform, ethylene chloride, tetrahydrofuran and dimethylformamide and optionally in the presence of a base such as sodium bicarbonate, triethyl amine, N,N-dimethylaniline. The temperature of the reaction may vary from -10° to 100° C., and the reaction time may vary from about 1/2 hour to 10 hours. The cephalosporin products are isolated by conventional methods.

The salt forms of Formula I wherein R₃ is a pharmaceutically acceptable cation are prepared in the manner recognized in the art and may be formed in situ or by reacting the corresponding acid with base, for example, sodium bicarbonate or triethylamine.

The compounds of Formula I wherein R₂ is selected from a hetero-arylthiol residue may also be prepared by the reaction of a compound of Formula I wherein R₂ is acetoxy namely Formula VI with an appropriate heteroarylthiol of Formula VII as schematically described in FIG. 1 ##STR10## wherein X, Y, W, Z, R₁, R₃ are as described above and R₁₃ S- is a heteroarylthio residue selected from 1,3,4-thiadiazol-2-ylthio; 5-methyl-1,3,4-thiadiazol-2-ylthio; tetrazol-2-ylthio; 1-methyltetrazol-5-ylthio; 1,3,4-oxadiazol-2-ylthio; 5-methyl-1,3,4-oxadiazol-2-ylthio; 1,3,4-triazol-2-ylthio; 5-methyl-1,3,4-triazol-2-ylthio; 1,2,3-triazol-5-ylthio.

The reaction is generally carried out in the presence of a solvent. Suitable solvents include water, methanol, ethanol, dimethylformamide, dimethylsulfoxide. The reaction is carried out by mixing in an appropriate solvent a compound of Formula VI with a compound of Formula VII, in such a way that the compound of Formula VII may be present in a non-stoichiometric excess relative to the compound of Formula VI. The reaction temperature may vary from about 25° C. to 100° C. and the reaction time may vary from about 0.5 hour to 10 hours. The reaction may be carried out in the presence of a base such as sodium carbonate, sodium bicarbonate, triethyl amine. The product of the reaction is isolated by conventional methods known in the art.

The individual optical isomers of the compounds of general Formula I wherein W represents methyl, amino, hydroxy, or SO₃ H are also included within the scope of this invention.

The novel compounds of this invention are useful as antibiotic agents as demonstrated by their activity against gram positive and gram negative bacteria in vitro and in vivo and fungi. The compounds of this invention are particularly useful in that they possess a longer duration of activity than many of the well known cephalosporin compounds. Illustrative examples of bacteria against which the compounds of this invention are active are Staphylococcus aureus, Salmonella schottmulleri, Klebsiella pneumoniae, Diplococcus pneumonia, and Streptococcus pyogenes.

The compounds of this invention may be administered alone or in the form of pharmaceutical preparations either orally, parenterally and topically. They may be administered to warm blooded animals, that is, birds and mammals, for example felines, canines, bovines, and equines, and humans. For oral administration the compounds may be administered in the form of tablets, capsules or pills or in the form of elixirs or suspensions. For parenteral administration they are best used in the form of a sterile aqueous solution which may contain other solutes, for example, enough saline or glucose to make the solution isotonic. For topical administration the compounds may be incorporated into creams or ointments.

GENERAL PROCEDURES FOR THE PREPARATION OF COMPOUNDS OF FORMULA I PROCEDURE I

To a solution of a compound of Formula II in 5-10 ml. of dimethylformamide an equimolar amount of a compound of Formula III is added. The solution is stirred at about 25° C. for 30 minutes and at 55° C. for three hours, and is then added to 200 ml of dichloromethane. The mixture is stirred for 30 minutes. The precipitated solid is filtered, washed with dichloromethane, and dried to give the product isolated as the hydrohalide salt.

PROCEDURE II

To a cold solution of sodium bicarbonate (2 equivalents) and a compound of Formula IV (1 equivalent) in a mixture of three parts of water to two parts of acetone, is added a compound of Formula V in one part of acetone. The solution is stirred for 30 minutes at about 25° C. and is then flash concentrated until all the acetone has been removed. To the obtained solution a compound of Formula III (1equivalent) is added. The solution is stirred at about 25° C. for two hours. During this time a solid precipitate is formed which is filtered, washed with water and dried to give the desired compound of Formula I.

EXAMPLE 1 3-[(Acetyloxy)methyl]-7-[[[(4-[[(5-amino-1,3,4-thiadiazol-2-yl)thio]methyl]-phenyl]acetyl]amino]-8-oxo-5-thia-1-aza-bicyclo[4.2.0]oct-2-ene-2-carboxylic acid hydrochloride.

The title compound is obtained in 56% yield when prepared according to Procedure I when the reagents used are 3-[(acetyloxy)methyl]-7-[[2-[4-(chloromethyl)phenyl]acetyl]amino]-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid (a compound of Formula II) and 5-amino-1,3,4-thiadiazol-2-thiol (a compound of Formula III).

NMR (DMSO-D₆ +D₂ O) ppm (δ), 2.03 (s, 3), 3.56 (broad s, 2), 4.38 (s, 2), 4.88 (q,2), 5.12 (d, 1), 5.70 (d, 1), 7.31 (s, 4).

Anal. Calcd' for C₂₁ H₂₁ N₅ O₆ S₃ HCl 2H₂ O: C, 41.47; H, 4.31; Cl, 5.83. Found: C, 41.31; H, 4.71; Cl, 5.84.

When in the procedure of Example 1 an appropriate amount of a chloromethyl substituted cephalosporin of Formula II listed in the following Table I is substituted for 3-[(acetyloxy)methyl]-7-[[2-[4-(chloromethyl)phenyl]acetyl]amino]-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid and is treated with an appropriate amount of a thiol compound of Formula III listed in the following Table I is substituted for 5-amino-1,3,4-thiadiazole-2-thiol, the respective 7-[[(substituted thio)methyl]phenyl]acetyl]cephalosporin product listed in Table I is obtained.

    __________________________________________________________________________     CHLOROMETHYL SUBSTITUTED                                                                               THIOL REAGENT                                          CEPHALOSPORIN DERIVATIVE                                                                               OF FORMULA III                                                                           SUBSTITUTED CEPHALOSPORIN IN                 __________________________________________________________________________                                       PRODUCT                                      3-[(acetyloxy)methyl]-7-[[2-[4-chloro-                                                                 4H-1,3,4-triazol                                                                         3-[(acetyloxy)methyl]-8-oxo-7-[[sulfo-       methyl)phenyl]-2-sulforacetyl]amino]-                                          2-ylthiol               [4-[(4H-1,3,4-triazol-2-ylthio)phenyl]                 8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-                                                                           acetyl]amino]-5-thia-1-azabicyclo[4.2.       ene-2-carboxylic acid             0]oct-2-ene-2-carboxylic acid                3-[(5-methyl-1,3,4-thiadiazol-2-ylthio)-                                                               methylthiol                                                                              7-[[carboxy[4-[(methylthio)methyl]-          methyl]-7-[[2-[4-(chloromethyl)phenyl]-                                                                          phenyl]acetyl]amino]-3-[[(5-methyl-          2-carboxyacetyl]amino]-8-oxo-5-thia-1-                                                                           1,3,4-thiadiazol-2-yl)thio]methyl]-8-        azabicyclo[4.2.0]oct-2-ene-2-carboxylic                                                                          oxo-5-thia-1-azabicyclo[4.2.0]oct-2-         acid                              ene-2-carboxylic acid                        3-[(5-methyl-1,3,4-thiadiazol-2-ylthio)-                                                               1H-tetrazol-                                                                             7-[[hydroxy[4-[(1H-tetrazol-5-ylthio)-       methyl]-7-[[3-[4-(chloromethyl)phenyl[-                                                                5-ylthiol methyl]phenyl]acetyl]amiono]-8-oxo-3-        2-hydroxyacetyl)amino)-8-oxo-5-thia-1-                                                                           [(5-methyl-1,3,4-thiadiazol-2-ylthio)-       azabicyclo[4.2.0]oct-2-ene-2-carboxylic                                                                          methyl]-5-thia-1-azabicyclo[4.2.0]           acid                              oct-2-ene-2-carboxylic acid                  3-[(acetyloxy)methyl]-7-[[2-[4-chloro-                                                                 5-methyl-1,3,4-                                                                          3-[(acetyloxy)methyl]-7-[[[[4-[[(5-          methyl)phenylthio]acetyl]amino]-8-oxo-                                                                 thiadiazol-2-                                                                            methyl-1,3,4-thiadiazol-2-yl)thio]-          5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-                                                                 ylthiol   methyl]phenyl]thio]acetyl]amino]-8-          carboxylic acid                   oxo-5-thia-1-azabicyclo[(4.2.0]oct-                                            2-ene-carboxylic acid                        3-methyl-7-[[2-[4-chloromethyl)phenyl]-                                                                5-methyl-1,3,4-                                                                          7-[[amino[4-[[(5-methyl-1,3,4-thia-          2-aminoacetyl]amino]-8-oxo-5-thia-1-                                                                   thiadiazol-2-yl-                                                                         diazol-2-yl)thio] 9 methyl]phenyl]-          azabicyclo[4.2.0]oct-2-ene-2-carboxylic                                                                thiol     acetyl]amino]-3-methyl-8-oxo-5-thia-         acid 1,1-dimethyl-2-oxopropoxymethyl                                                                             1-azabicyclo[4.2.0]oct-2-ene-2-              ester                             carboxylic acid, (1,1-dimethyl-2-                                              oxopropoxy)methyl ester                      3-[(1-methyltetrazol-5-ylthio)methyl]-                                                                 5-amino-1,3,4-                                                                           7-[[[4-[[(5-amino-1,3,4-thiadiazol-          7-[[2-[4-[(chloromethyl)phenyl]acetyl]-                                                                thiadiazol-2-                                                                            2-yl)thio]methyl]phenyl]acetyl]-             amino]-8-oxo-5-thia-1-azabicyclo[4.2.0]                                                                ylthiol   amino]-8-oxo-3-[(1-methyltetrazol-           oct-2-ene-carboxylic acid         5-ylthio) methyl]-5-thia-1-azabicyclo-                                         [4.2.0]oct-2-ene-2-carboxylic acid           7-[[2-[4-(chloromethyl)phenyl]acetyl]-                                                                 1-methyl-1H-                                                                             3-methyl-7-[[[4-[[(1-methyl-1H-tetra-        amino]-3-methyl-8-oxo-5-thia-1-aza-                                                                    1-tetrazol-5-                                                                            zol-5-yl)thio]methyl]phenyl]acetyl]-         bicyclo[4.2.0]oct-2-ene-2-carboxylic                                                                   ylthiol   amino]-8-oxo-5-thia-1-azabicyclo-            acid                              [4.2.0]oct-2-ene-2-carboxylic                __________________________________________________________________________                                       acid                                    

EXAMPLE 2 7[[[4-[[(5-Amino-1,3,4-thiadiazol-2-yl)-thio]methyl]-phenyl]acetyl]amino]-3-[[(1-methyl-1H-tetrazol-5-yl)thio]-methyl]-8-oxo-5-thia-1-azabicyclo[4.2.0] oct-2-ene-2-carboxylic acid.

The title compound is obtained in 44% yield when prepared according to Procedure II, when the reagents used are 3-(1-methyl]tetrazolyl-5-thio)-7-amino-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid (a compound of Formula IV) p-chloromethylphenylacetyl chloride (a compound of Formula V) and 5-amino-1,3,4-thiadiazole-2-thiol (a compound of Formula III).

NMR(DMSO-D₆ +D₂ O), ppm (δ), 3.55 (broad s, 2), 3.97 (s, 3), 4.20 (broad s, 2), 5.10 (d, 1), 5.70 (d, 1), 7.3 (s, 4).

EXAMPLE 3 3-[[(1-Methyl-1H-tetrazol-5 -yl)thio]-methyl]-7-[[4-[[(1-methyl-1H-tetrazol-5-yl)-thio]methyl]phenyl]-acetyl]amino]-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid.

The title compound is obtained in 59% yield as described in Example 2 when an appropriate amount of 1-methyl-1,2,3,4-tetrazole-5-thiol is used instead of 5-amino-1,3,4-thiadiazole-2-thiol.

NMR (DMSO-D₆ +D₂ O), ppm (δ), 3.45 (broad s, 2), 3.73 (s, 3), 3.87 (s, 3), 4.42 (broad s, 2), 5.0 (d, 1), 5.6 (d, 1), 7.2 (s, 4).

EXAMPLE 4 3-[[(1-Methyl-1H-tetrazol-5-yl)thio]methyl-8-oxo-7-[[[4-[](1H-1,2,4-triazol-3-yl)thio]-methyl]phenyl]acetyl]amino]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid.

The title compound is obtained in 43% yield as described in Example 2 when an appropriate amount of 1,2,4-triazole-5-thiol is used instead of 5-amino-1,3,4-thiadiazole-2-thiol.

NMR (DMSO-D₆ +D₂ O), ppm (δ), 3.44 (broad s, 2), 3.88 (s, 3), 4.25 (broad s, 2), 5.0 (s, 1), 5.6 (s, 1), 7.2 (s, 4), 8.0 (s, 1).

EXAMPLE 5 3-[(Acetyloxy)methyl]-7-[[[4-[[(4,5-dihydro-4-oxo-2-thiazolyl)thio]methyl]phenyl]acetyl]amino]-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid.

The title compound is obtained in 47% yield when prepared according to Procedure I when the reagents used are 3-[(acetyloxy)methyl]-7-[[2-[4-(chloromethyl)phenyl]-2-ene-2-carboxylic acid (a compound of Formula II) and (4,5-dihydro-4-oxo-2-thiazolyl)thiol (a compound of Formula III).

NMR (DMSO-D₆ +D₂ O) ppm (δ), 3.58 (broad s, superimposed two s,4), 4.5-5.3 (broad, m. 7), 5.71 (d, 1), 7.1-7.5 (m, 4).

EXAMPLE 6 3-[(Acetyloxy)methyl]-8-oxo-7-[[[4-(thiocyanatomethyl)phenyl]acetyl]amino]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid.

The title compound is obtained in 55% yield as described in Example 1 when an appropriate amount of potassium thiocyanate is used instead of 5-amino-1,3,4-thiadiazole-2-thiol.

NMR (DMSO-D₆ +D₂ O) ppm (δ) 2.02 (s,3), 3.5 (brad band, 4), 4.3 (s,2), 4.9 (q,2), 5.05 (d,1), 5.64 (d,1), 7.37 (s, 4). 

We claim:
 1. A compound of the formula ##STR11## wherein R is selected from the group consisting of a lower alkyl group of from 1 to 4 carbon atoms, 1,3,4-thiadiazol-2-ylthio, 5-methyl-1,3,4-thiadiazol-2-ylthio, tetrazol-5-ylthio, 1-methyltetrazol-5-ylthio, 1,3,4-oxadiazol-2-ylthio, 5-methyl-1,3,4-oxadiazol-2-ylthio, 1,3,4-triazol-2-ylthio, 5-methyl-1,3,4-triazol-2-ylthio, 1,2,3-triazol-5-ylthio, 5-amino-1,3,4-thiadiazol-2-ylthio, (4,5-dihydro-4-oxo-2-thiazolyl)thio or cyano; Y is selected from the group consisting of hydrogen, chlorine, bromine, a straight or branched lower alkyl group of from 1 to 4 carbon atoms, or an alkoxy group of from 1 to 4 carbon atoms; Z is a bond, oxygen or sulfur; W is selected from the group consisting of hydrogen, methyl, amino, hydroxy, SO₃ H, or COOR₄ wherein R₄ is hydrogen or 5-indanyl with the proviso that when Z is oxygen or sulfur, W is other than hydroxy; R₁ is hydrogen or methoxy; R₂ is hydrogen or acetoxy; R₃ is selected from the group consisting of hydrogen, a cation of an alkali metal or an alkaline earth metal, ammonium or organic ammonium cations, a straight or branched lower alkyl group of from 1 to 4 carbon atoms, an alkoxycarbonylaminomethyl group in which the alkoxy moiety is straight or branched and has from 1 to 4 carbon atoms and the amine nitrogen may be substituted with a straight or branched lower alkyl group in which the alkanoyl moiety is straight or branched and has from 2 to 5 carbon atoms, an aminoalkanoyloxymethyl group in which the alkanoyl moiety has from 2 to 15 carbon atoms and the amino nitrogen may be mono- or di-substituted with a straight or branched lower alkyl group having from 1 to 4 carbon atoms, alkanoyloxymethyl in which the alkanoyl moiety is straight or branched and has from 2 to 5 carbon atoms, alkanoylaminomethyl in which the alkanoyl moiety is straight or branched and has from 2 to 5 carbon atoms and p-(alkanoyloxy)benzyl in which the alkanoyl moiety is straight or branched and has from 2 to 5 carbon atoms; and pharmaceutically acceptable salts and individual optical isomers thereof.
 2. A compound of claim 1 wherein W is selected from hydrogen, amino, CO₂ H, SO₃ H.
 3. A compound of claim 2 wherein Z is a bond.
 4. A compound of claim 3 wherein Y is hydrogen.
 5. A compound of claim 4 which is 3-[(acetyloxy)methyl]-7-[[[(4-[[(5-amino-1,3,4-thiadiazol-2-yl)thio]-methyl]-phenyl]acetyl]amino]-8-oxo-5-thia-1-azabicyclo-4.2.0]oct-2-ene-2-carboxylic acid or a pharmaceutically acceptable salt thereof.
 6. A compound of claim 4 which is 3-[(acetyloxy)-methyl]-7-[[[4-[[(4,5-dihydro-4-oxo-2-thiazolyl)thio]methyl]phenyl]acetyl]amino ]-8-oxo-5-thia-1-azabicyclo-[4.2.0]oct-2-ene-2-carboxylic acid or a pharmaceutically acceptable salt thereof.
 7. A compoundof claim 4 which is 3-[(acetyloxy)methyl[-8-oxo-7-[[[4-(thiocyanatomethyl)phenyl]acetyl]-amino]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid or a pharmaceutically acceptable salt thereof. 